组织样品检测
胸腺：研究T细胞分化、发育；
淋巴结：T细胞、B细胞、DC细胞分析；
骨髓：B细胞发育研究，红细胞发育研究，造血干细胞研究；
肾组织：分析炎性细胞浸润；
肺组织：小鼠肺组织中免疫细胞组成分析，肿瘤或炎症小鼠肺组织中免疫细胞浸润分析；
皮肤：细胞炎性浸润分析；
脑组织：分析组织中炎性细胞浸润情况。
实验案例一：胸腺T细胞发育
Population | Gating Step | ||||
Th Cells | Live single | mCD4+ | mCD8- | / | / |
Tc Cells | Live single | mCD4- | mCD8+ | / | / |
DP Cells | Live single | mCD4+ | mCD8+ | / | / |
DN Cells | Live single | mCD4- | mCD8- | / | / |
DN2 Cells | Live single | mCD4- | mCD8- | mCD44+ | mCD25+ |
DN3 Cells | Live single | mCD4- | mCD8- | mCD44- | mCD25+ |
DN1 Cells | Live single | mCD4- | mCD8- | mCD44+ | mCD25- |
DN4 Cells | Live single | mCD4- | mCD8- | mCD44- | mCD25- |
实验案例二：肾脏免疫细胞分析（12色方案）
Population | Gating Step | ||||
Total Leukocytes | Live single | mCD45+ | / | / | / |
Neutrophiles | Live single | mCD45+ | mCD11b+ | Ly6G+ | / |
Monocytes | Live single | Not Neutrophiles | mCD11b+ | Ly6C hi | / |
Eosinophils | Live single | Not monocytes | mCD11b+ | SSC-H hi | / |
Macrophage | Live single | Not eosinophils | mCD11b+ | F4/80+ | / |
DC Cells | Live single | Not eosinophils | mCD11b+ | mCD11c+ | / |
T Cells | Live single | Not eosinophils | mCD3+ | mCD335- | / |
B Cells | Live single | Not eosinophils | mCD19+ | / | / |
NK Cells | Live single | Not eosinophils | mCD3- | mCD335+ | / |
Th Cells | Live single | Not eosinophils | mCD3+ | mCD4+ | mCD8- |
Tc Cells | Live single | Not eosinophils | mCD3+ | mCD4- | mCD8+ |
实验案例三：脑组织浸润免疫细胞分析（10色方案）
Population | Gating step | ||||
Total Leukocytes | Live single | mCD45+ | / | / | / |
Neutrophiles | Live single | mCD45+ | mCD11b+ | Ly6G+ | / |
Monocytes | Live single | Not Neutrophiles | mCD11b+ | Ly6C hi | / |
Eosinophils | Live single | Not monocytes | mCD11b+ | SSC-H hi | / |
Macrophage | Live single | Not eosinophils | mCD11b+ | F4/80+ | / |
T cells | Live single | Not eosinophils | mCD3+ | mCD19- | / |
B cells | Live single | Not eosinophils | mCD19+ | mCD3- | / |
Th | Live single | Not eosinophils | mCD3+ | mCD4+ | mCD8- |
Tc | Live single | Not eosinophils | mCD3+ | mCD4- | mCD8+ |
药康优势：
1. 拥有丰富的组织检测项目经验，不同组织有特异性的消化处理方法；
2. 提供常规流式检测Panel，同时可以根据药物特点，针对性的检测靶点或独特的细胞群体，提供定制化服务；
3. 严格的实验室数据保存方案，实验数据具有可追溯性 ，满足新药申报合规性要求。